Ectopic replantation of forearm, wrist and palm in a destructive injury of a young child: A case report / 中华显微外科杂志

Reports a case admitted in the Ward I of Department of Surgery of Zhengzhou Renji Hospital in June 2017. A young child who suffered destructive injury of left forearm, wrist and palm with severed 3rd-5th fingers. Tendon and neurovascular repairs of forearm, wrist and palm were performed with pedicled abdomina flap and the 3rd-5th fingers ectopic replantation in Phase I surgery. In the Phase II surgery, the abdomina flap division was carried out. The replantation of severed fingers after ectopic replantation and the reconstruction of foot defect with free anterolateral thigh flap(ALTF) were carried out in Phase III surgery. In Phase IV surgery, fingers functional reconstruction and foot flap thinning were performed. Four years after surgery, the thumb oppositions to middle, ring and little fingers could be completed, with slightly limitations. The appearance and texture of transferred foot flap were good, and the child could walk and run almost normally.

Expression of estrogen receptor alpha protein and BRAF V600E protein in thyroid papillary carcinoma and its clinical significance / 中华普通外科杂志

Objective:To investigate the expression of estrogen receptor alpha (ERα) protein and BRAF V600E protein in thyroid papillary carcinoma (PTC) and their relationship with clinical factors of PTC. Methods:The expression of ERα and BRAF V600E protein in 1 105 PTC patients was detected by immunohistochemistry. The relationship among ERα, BRAF V600E protein and clinical factors were analyzed. Results:Positive ERα protein was correlated with maleness (χ 2= 6.087, P=0.001), age< 45 years old (χ 2=5.197, P=0.023) and multifocal tumors (χ 2=4.446, P=0.035). Positive BRAF V600E protein was correlated with positive ERα protein (χ 2=6.209, P=0.013), Hashimoto thyroiditis (χ 2=29.388, P<0.001), no lateral lymph node metastasis (χ 2=6.849, P=0.009) and multifocal tumors (χ 2=9.596, P=0.035). Conclusions:ERα expression is more common in male patients, patients younger than 45 years of age, those with multifocal tumors and positive BRAF V600E protein. BRAF V600E protein may inhibit Hashimoto's thyroiditis, tumor growth and the occurrence of lateral lymph node metastasis, and promote the occurrence of multiple focal tumors.

A Fluorescence Biosensor for Detection of Mercury Ion Based on Oligonucleotide / 分析化学

A simple,fast and highly sensitive fluorescence analysis method for detection of mercury ion was developed based on N-methyl-mesoporphyrin IX (NMM)/G-quadruplex DNA system and specific T-Hg-T mismatches.In this strategy,a large number of thymine was introduced into guanine-rich oigonucleotides which could form G-quadruplex.In the presence of Hg2+,guanine-rich oigonucleotides and complementary strand could form double-stranded DNA molecule by specific T-Hg-T mismatch pair,leading to destruction of G-quadruplex DNA structure.In the absence of Hg2+,guanine-rich oigonucleotides spontaneously formed G-quadruplex DNA structure that could bound NMM to generate intense fluorescence.Based on the above facts,a sensitive fluorescence biosensor for determination of Hg2+ was fabricated.And the optimal conditions for Hg2+ determination were as follows:buffer solution pH of 6.7,20 mmol/L KCl and 2.5 μmol/L NMM in buffer and incubation for 2 h.Under the optimal conditions,the fluorescence intensity signal change (F0-F) and the Hg2+ concentration exhibited a linear correlation within 50 nmol/L to 1000 nmol/L range with a low detection limit of 22.8 nmol/L (3σ).The biosensor exhibited good selectivity toward common metal ions.The developed method was successfully employed to detect Hg2+ in tap water with recovery of 106.1％-107.8％.

Process development for continuous ethanol fermentation by the flocculating yeast under stillage backset conditions / 生物工程学报

Propionic acid, a major inhibitor to yeast cells, was accumulated during continuous ethanol fermentation from corn meal hydrolysate by the flocculating yeast under stillage backset conditions. Based on its inhibition mechanism in yeast cells, strategies were developed for alleviating this effect. Firstly, high temperature processes such as medium sterilization generated more propionic acid, which should be avoided. Propionic acid was reduced significantly during ethanol fermentation without medium sterilization, and concentrations of biomass and ethanol increased by 59.3% and 7.4%, respectively. Secondly, the running time of stillage backset should be controlled so that propionic acid accumulated would be lower than its half inhibition concentration IC50 (40 mmol/L). Finally, because low pH augmented propionic acid inhibition in yeast cells, a higher pH of 5.5 was validated to be suitable for ethanol fermentation under the stillage backset condition.

Effect of adriamycin nanoparticles conjugated with hyaluronic acid on oral squamous cell carcinomas in vitro / 中国组织工程研究

ACKGROUND: Adriamycin (ADM) can specifically conjugate with receptor. In particular, ADM nanopartides play a target role in decreasing the cytotoxicity.OBJECTIVE: To investigate the targeting effect of addamycin nanoparticles conjugated with hyaluronic acid (ADM-HA-SSL) on oral squamous carcinomas calls in vitro.DESIGN, TIME AND SETTING: An in vitro contrast observation was performed in College of Madne Life Science, Ocean University of China from January to July 2008. MATERIALS: Oral squamous carcinomas calls were sincerely presented by Professor Chen from the Ninth People's Hospital of Shanghai; ADM-HA-SSL (drug loading 156 mg/L) was sincerely presented by Professor Liu from College of Marine Life Science, Ocean University of Chine.METHODS: Oral squamous carcinomas cells were cultured with 0.5, 1.0, 5.0, and 10.0 mg/L ADM-HA-SSL. MTT assay was used to detect the targeted cytotoxicity of ADM-HA-SSL against oral squamous cell carcinomas. With the concentrations of 5.0 and 10.0 mg/L, call apoptosis was ascertained by call flow cytometry after 6, 12, 24 and 48 hours. MAIN OUTCOME MEASURES: Cell survival rate and apoptosis rate.RESULTS: At 24 and 48 hours after induction, cytotoxicity assay revealed that the effect of ADM-HA-SSL was superior to that of free ADM (t=5.78-42.05, P < 0.01). The results of flow cytometry showed that the apoptosis rate was enhanced with the increase of the time (F=4 200.40, 4 775.36, P < 0.01), and the rate was also increased at the same time point with the increase ofconcentration (t=12.06-20.08, P < 0.05).CONCLUSION: ADM-HA-SSL can specifically recognize oral squamous carcinomas cells and deliver adriamycin into the cells. And the effect is enhanced by the time prolonging.

Design and Implementation of A Portable ECG Monitor for Multi-user / 航天医学与医学工程

Objective To design and implement a portable electrocardiogram (ECG) monitor for multiple users. Methods Multilevel menu on liquid crystal displayer(LCD) for switching among users based on digital signal processor(DSP) was developed. Record header in ECG records for different users was appended, and communication protocol between monitor and hospital center was made. Results This monitor could record three users’s ECG signal and transmit the ECG records to remote hospital center via digital communication method. The hospital center could receive and file the ECG records of different users. Conclusion This monitor can be used for three users and is more efficient in the application of ECG monitoring.

The sudies on the permeability property of chitosan membranes / 中国海洋药物

Chitosan, a linear polysaccharide, is obtained by chitin N deacetylation. Its ?(1 4) glucosidic bond is not stable and breaks easily in acid condition. In this study, chitosan of different molecular weights(MW) was prepared by acetic acid degradation. Chitosan was dissolved at 3%(w/v) concentration in 1%(v/v) acetic acid and the solution was dried at 50 degree C. We get chitosan membranes of different MW after neutralization with aqueous NaOH. The membranes are transparent, elastic and have good intensity. The permeability studies on chitosan membranes were going on with a permeable install made by our laboratory. It was determined by the transmission concentration of NaCl, L Tyr, glucose and calf albumin. We compared the permeability property with different MW chitosan membranes. The result implied that all of the four samples could permeate through the chitosan membranes. And the permeability property of the membrane increased with the chitosan MW decreaseing.

Screening of a high yield protease producing strain and studies on its fermentation conditions / 中国海洋药物

A1 strain with fibrinolytic activity was isolated from sea water in Qingdao. After combination treatment with ultraviolet light and MNNG, a mutant C22 producing 2947.60 u?mg -1 protein of fibrinolytic enzyme in culture broth was obtained.Its enzyme activity was 4.23 fold of wild stain A1.The optimum medium for fermentation consisted of 2.5% soy bean cake meal, 0.1% yeast extract,0.2%NaCl,0.05%MgSO 4?7H 2O,0.001%FeSO 4?7H 2O.They were dissolved with 0.05mol?L -1 ,pH7.4 phosphoric buffer.The strain producing maximum fibrnolytic activity after growth at 25℃ for 40h on a rotary shaker.The initial optimum pH was 7.0~7.5.